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Performance Evaluation of Blended Activated Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater

Received: 21 April 2026     Accepted: 24 July 2026     Published: 10 August 2026
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Abstract

The discharge of untreated abattoir wastewater poses a significant environmental and public health challenge due to its high concentrations of organic matter, suspended solids, nutrients, and other pollutants. Conventional treatment methods are often expensive and difficult to sustain in developing countries, creating the need for affordable and environmentally friendly alternatives. This study investigated the effectiveness of blended activated charcoal and calcium carbonate (AC–CaCO3) as a low-cost adsorbent for the treatment of abattoir effluent. Wastewater samples were collected from the Warake Road abattoir in Auchi, Edo State, Nigeria, and treated using AC–CaCO3 blends prepared in ratios of 1:1, 1:2 and 2:1. The treatment performance was evaluated by analysing physicochemical parameters, including pH, temperature, total suspended solids (TSS), total dissolved solids (TDS), turbidity, biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), nitrate, phosphate, and selected heavy metals. The results showed that the adsorption efficiency depended on the blending ratio, with the 1:2 (AC–CaCO3) blend exhibiting the best overall performance. This blend achieved removal efficiencies of 81% for BOD, 83% for COD, 82% for TSS, 72% for nitrate, and 68% for phosphate. Furthermore, the pH of the treated effluent was stabilised within the range of 6.7–7.7, while dissolved oxygen increased significantly from 0.8 to 4.3 mg/L, indicating substantial improvement in water quality. The treated effluent approached the permissible limits specified by the World Health Organization (WHO) and the Nigerian Industrial Standards (NIS) for wastewater discharge. The findings demonstrate that blended AC–CaCO3 is an effective, economical, and environmentally sustainable adsorbent for the treatment of abattoir wastewater and offers a promising solution for improving wastewater management in resource-constrained settings.

Published in Journal of Energy, Environmental & Chemical Engineering (Volume 11, Issue 2)
DOI 10.11648/j.jeece.20261102.12
Page(s) 55-60
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2026. Published by Science Publishing Group

Keywords

Abattoir Wastewater, Activated Charcoal, Calcium Carbonate, Adsorption, Effluent Treatment, Water Quality, Sustainable Engineering

1. Introduction
The rapid expansion of livestock processing in developing regions has intensified the release of untreated abattoir effluent into the environment. Abattoir wastewater typically contains blood, fats, soluble proteins, undigested matter, and fecal residues that generate excessive organic and nutrient loads . Such effluents exhibit high biochemical oxygen demand (BOD) and chemical oxygen demand (COD), resulting in oxygen depletion and eutrophication when discharged into natural watercourses .
In Nigeria, most abattoirs lack adequate wastewater treatment systems. Facilities such as the Warake Road abattoir in Auchi discharge effluents directly into open drains, causing severe contamination of surface and groundwater. This condition leads to ecosystem degradation, biodiversity loss, and public-health risks for surrounding communities . Conventional treatment systems like aeration, coagulation, and biological oxidation are often cost-intensive, complex, and unsustainable for small-scale operations. There is, therefore, a need for locally sourced, low-cost treatment technologies .
Adsorption has emerged as a versatile technique capable of removing organic, inorganic, and microbial pollutants through surface interaction between contaminants and adsorbent particles . Locally available materials such as activated charcoal and calcium carbonate are gaining attention for their affordability and environmental compatibility. Activated charcoal, produced from biomass residues, offers high surface area and porosity, while calcium carbonate provides buffering and coagulating effects. Their combination as a blended filler produces synergistic behavior that enhances pollutant removal .
Previous studies have confirmed the improved efficiency of hybrid adsorbents through the combined effects of adsorption and precipitation . However, research on AC–CaCO3 blends for abattoir wastewater treatment remains limited, particularly under field conditions typical of Nigerian abattoirs. This study addresses this gap by developing and testing blended AC–CaCO3 fillers and evaluating their treatment performance .
Accordingly, the objective is to evaluate the treatment efficiency of blended activated charcoal–calcium carbonate fillers in improving abattoir wastewater quality. The study involves:
1) Collection and characterization of raw wastewater from Warake Road, Auchi;
2) Preparation of AC–CaCO3 blends in ratios 1:1, 1:2, and 2:1;
3) Assessment of pollutant removal performance for major parameters; and
4) Comparison of treated effluent quality with WHO and NIS standards.
2. Materials and Methods
2.1. Study Area
The study was conducted at the Warake Road abattoir, Auchi, Edo State, Nigeria. The abattoir lacks a formal wastewater treatment system and discharges directly into open drains leading to nearby streams.
2.2. Effluent Sampling
Samples were obtained during peak operations (08:00–11:00 am) using sterilized 2-L polyethylene containers pre-washed with nitric acid and distilled water. Three samples were composited and preserved at 4°C before analysis, following APHA Standard Methods .
Figure 1. Sample Used.
2.3. Materials and Reagents
Activated Charcoal (AC): Prepared from hardwood sawdust carbonized at 600°C for 2 h and sieved to 150 µm.
Calcium Carbonate (CaCO3): Derived from natural limestone, washed, oven-dried at 105°C, and sieved to 150 µm.
Analytical Reagents: Potassium dichromate, ferrous ammonium sulfate, and concentrated sulfuric acid.
Equipment: pH meter (Hanna HI2211), UV–Vis spectrophotometer (Hach DR 3900), and Atomic Absorption Spectrophotometer (PerkinElmer AAnalyst 400).
2.4. Preparation of Blended Fillers
Three blends were prepared by weight: Sample A (1:1), Sample B (1:2), and Sample C (2:1). Each mixture was homogenized using a mechanical stirrer and stored in airtight containers.
2.5. Treatment Procedure
For each test, 200 mL of effluent was treated with 5 g of blended filler in a 500 mL beaker. The mixture was stirred at 200 rpm for 30 min, allowed to settle for 24 h, and filtered. Control (untreated) samples were analyzed concurrently.
Figure 2. Blended Activated Charcoal–Calcium Carbonate.
2.6. Analytical Methods
Table 1. Analytical Methods.

Parameter

Method

Reference

pH, Temperature

Electrometric

APHA (2017)

TSS, TDS

Gravimetric

APHA (2017)

BOD

Winkler method

APHA (2017)

COD

Dichromate oxidation

APHA (2017)

Nitrates/Phosphates

Spectrophotometric

APHA (2017)

Heavy Metals

AAS

ISO 8288 (1986)

2.7. Data Analysis
All experiments were conducted in triplicate. Removal efficiency was computed as:
Ci-CfCi x 100(1)
where Ci and Cf are the initial and final concentrations, respectively. Significance was evaluated at p < 0.05 using ANOVA.
3. Results and Discussion
3.1. Physicochemical Characterization of Raw Effluent
The untreated effluent exhibited a dark red-brown color, strong odor, and high turbidity due to suspended organic matter and fats. Table 2 summarizes the raw effluent characteristics, confirming severe contamination and the need for effective treatment.
Table 2. Physicochemical Characteristics of Raw Abattoir Effluent.

Parameter

Observed Value

WHO/NIS Limit

Remarks

pH

6.7

6.0–9.0

Slightly acidic

Temperature (°C)

28

< 30

Within range

BOD (mg/L)

360

< 30

Excessively high

COD (mg/L)

1050

< 250

Excessive organic load

TSS (mg/L)

500

< 30

Poor clarity

TDS (mg/L)

650

< 500

High dissolved solids

Nitrate (mg/L)

24

< 10

Above limit

Phosphate (mg/L)

19

< 5

Above limit

DO (mg/L)

0.8

> 4.0

Oxygen deficient

These results align with earlier studies reporting high organic and nutrient pollution in abattoir effluents .
3.2. Effect of Treatment on Oxygen Demand
The 1:2 AC–CaCO3 blend achieved the highest reduction in BOD and COD. This improvement is attributed to the combined adsorption of organic matter by activated charcoal and oxidation catalysis promoted by calcium carbonate.
Table 3. Oxygen Demand Parameters Before and After Treatment.

Parameter

Raw

1:1

1:2

2:1

WHO Limit

BOD (mg/L)

360

310

68

300

<30

COD (mg/L)

1050

920

180

880

<250

DO (mg/L)

0.8

1.2

4.3

2.0

>4.0

The 1:2 blend reduced BOD and COD by 81% and 83%, respectively, and increased DO by over 400%.
3.3. Removal of Suspended and Dissolved Solids
The 1:2 ratio produced an 82% TSS reduction and 70% TDS reduction, enhancing clarity and reducing turbidity. The synergistic effect between adsorption and precipitation explains the improved removal efficiency.
Table 4. Removal Efficiency for Suspended Solids and Dissolved Substances.

Parameter

Raw (mg/L)

Treated (1:2) (mg/L)

Removal Efficiency (%)

TSS

500

90

82

TDS

650

195

70

Turbidity (NTU)

118

20

83

3.4. Nutrients and Heavy Metal Removal
Nitrate and phosphate concentrations dropped by 72% and 68%, respectively, due to adsorption and ionic interaction with calcium ions. Heavy metals exhibited strong affinity to the carbon surface, with lead showing the highest removal (79%).
Table 5. Nutrient and Heavy Metal Removal Efficiencies for 1:2 Blend.

Parameter

Raw (mg/L)

Treated (mg/L)

Removal Efficiency (%)

Nitrate (NO₃⁻)

24

6.7

72

Phosphate (PO₄³⁻)

19

6.1

68

Lead (Pb)

0.33

0.07

79

Zinc (Zn)

0.42

0.13

70

Copper (Cu)

0.31

0.08

73

Chromium (Cr)

0.27

0.10

63

Cadmium (Cd)

0.15

0.05

65

The improved performance demonstrates the ability of the blended filler to simultaneously reduce nutrient enrichment and metal toxicity.
3.5. Microbial Reduction
The total coliform count decreased from 1.2×103 to 1.8×102 CFU/mL, corresponding to 85% removal. The alkaline buffering of CaCO3 and adsorptive surfaces of activated charcoal reduced microbial load, improving effluent hygiene .
3.6. Overall Performance Evaluation
The 1:2 AC–CaCO3 blend consistently produced the highest pollutant reduction across all tested parameters.
Table 6. Summary of Optimum Treatment Performance.

Parameter

Removal Efficiency (%)

BOD

81

COD

83

TSS

82

TDS

70

Nitrate

72

Phosphate

68

Pb

79

Cu

73

Zn

70

Cd

65

Cr

63

Coliforms

85

This confirms that the 1:2 ratio ensures an ideal balance between adsorptive surface area and buffering capacity.
4. Conclusion and Engineering Implications
The blended activated charcoal–calcium carbonate adsorbent demonstrated strong potential for abattoir wastewater treatment. The 1:2 ratio achieved optimal removal efficiency for organic matter, solids, nutrients, and metals, bringing effluent quality close to WHO and NIS discharge limits. The method is low-cost, eco-friendly, and suitable for small-scale abattoirs.
Engineering implications include modular deployment of AC–CaCO3 filters in decentralized wastewater systems, minimal operation cost, and potential integration into sustainable abattoir infrastructure.
Abbreviations

BOD

Biochemical Oxygen Demand

COD

Chemical Oxygen Demand

Acknowledgments
The authors would like to thank the Department of Civil Engineering, Edo State University, Iyamho, Nigeria, for providing the facilities used in conducting this research. Appreciation is also extended to the laboratory staff for their technical support.
Author Contributions
Eshiole
Ibrahim Abdulrazaq Olayinka: Validation
Wasiu John: Supervision
Sule Joseph: Visualization
Conflicts of Interest
The authors declare no conflict of interest.
References
[1] A. A. Ogundipe, I. A. Bello, and C. O. Akinbile, “Pollution load assessment of abattoir effluent on surface water quality,” Environmental Monitoring and Assessment, vol. 190, pp. 680, 2018.
[2] C. O. Akinbile, T. O. Ogunbode, and O. A. Oladeji, “Characterization and treatment of abattoir effluent in Nigeria: A review,” Environmental Technology & Innovation, vol. 22, pp. 101440, 2021.
[3] M. S. Ahmed, K. Nwankwo, and A. Bello, “Performance of blended adsorbents in industrial effluent management,” Water Science & Technology, vol. 81, no. 9, pp. 1889–1901, 2020.
[4] R. Singh, D. Kumar, and P. Mehta, “Activation and modification of calcium carbonate for enhanced adsorption,” Applied Water Science, vol. 9, p. 192, 2019.
[5] A. O. Adesola, A. O. Ogunbayo, and O. P. Ajayi, “Low-cost adsorbents for wastewater treatment: A review,” Environmental Engineering Research, vol. 27, no. 5, pp 220046, 2022.
[6] A. A. Mahmoud, D. O. Nwude, and C. O. Ikeh, “Enhancing pollutant adsorption using mineral–carbon blends,” Applied Water Science, vol. 10, p. 245, 2020.
[7] APHA, Standard Methods for the Examination of Water and Wastewater, 23rd ed., Washington, DC: American Public Health Association, 2017.
[8] M. Ali, R. Hussain, and A. Malik, “Characterization and treatment of abattoir wastewater: A case study,” Journal of Environmental Protection, vol. 12, pp. 210–225, 2021.
[9] W. Nafarnda, O. Okolocha, and E. Inyang, “Microbial contamination of abattoir effluent and environmental impact,” African Journal of Environmental Science, vol. 14, pp. 101–110, 2020.
[10] J. Vymazal, “Constructed wetlands for wastewater treatment: Five decades of experience,” Environmental Science and Policy, vol. 106, pp. 36–44, 2020.
[11] Chukwu, O. (2008). Analysis of groundwater pollution from abattoir waste in Minna, Nigeria. Research Journal of Dairy Sciences, 2(4), 74–77.
[12] Metcalf & Eddy, Inc. (2014). Wastewater engineering: Treatment and resource recovery (5th ed.). McGraw-Hill Education.
[13] Adeyemo, O. K. (2002). Unhygienic operation of a city abattoir in South Western Nigeria: Environmental implication. African Journal of Environmental Assessment and Management, 4(1), 23–28.
[14] Foo, K. Y., & Hameed, B. H. (2010). Insights into the modeling of adsorption isotherm systems. Chemical Engineering Journal, 156(1), 2–10.
[15] Bhatnagar, A., Vilar, V. J. P., Botelho, C. M. S., & Boaventura, R. A. R. (2011). A review of the use of red mud as adsorbent for the removal of toxic pollutants from water and wastewater. Environmental Technology, 32(3), 231–249.
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    Oshiobugie, E. L., John, W., Olayinka, I. A., Joseph, S. (2026). Performance Evaluation of Blended Activated Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater. Journal of Energy, Environmental & Chemical Engineering, 11(2), 55-60. https://doi.org/10.11648/j.jeece.20261102.12

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    ACS Style

    Oshiobugie, E. L.; John, W.; Olayinka, I. A.; Joseph, S. Performance Evaluation of Blended Activated Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater. J. Energy Environ. Chem. Eng. 2026, 11(2), 55-60. doi: 10.11648/j.jeece.20261102.12

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    AMA Style

    Oshiobugie EL, John W, Olayinka IA, Joseph S. Performance Evaluation of Blended Activated Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater. J Energy Environ Chem Eng. 2026;11(2):55-60. doi: 10.11648/j.jeece.20261102.12

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  • @article{10.11648/j.jeece.20261102.12,
      author = {Eshiole Lucky Oshiobugie and Wasiu John and Ibrahim Abdulrazaq Olayinka and Sule Joseph},
      title = {Performance Evaluation of Blended Activated 
    Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater},
      journal = {Journal of Energy, Environmental & Chemical Engineering},
      volume = {11},
      number = {2},
      pages = {55-60},
      doi = {10.11648/j.jeece.20261102.12},
      url = {https://doi.org/10.11648/j.jeece.20261102.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jeece.20261102.12},
      abstract = {The discharge of untreated abattoir wastewater poses a significant environmental and public health challenge due to its high concentrations of organic matter, suspended solids, nutrients, and other pollutants. Conventional treatment methods are often expensive and difficult to sustain in developing countries, creating the need for affordable and environmentally friendly alternatives. This study investigated the effectiveness of blended activated charcoal and calcium carbonate (AC–CaCO3) as a low-cost adsorbent for the treatment of abattoir effluent. Wastewater samples were collected from the Warake Road abattoir in Auchi, Edo State, Nigeria, and treated using AC–CaCO3 blends prepared in ratios of 1:1, 1:2 and 2:1. The treatment performance was evaluated by analysing physicochemical parameters, including pH, temperature, total suspended solids (TSS), total dissolved solids (TDS), turbidity, biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), nitrate, phosphate, and selected heavy metals. The results showed that the adsorption efficiency depended on the blending ratio, with the 1:2 (AC–CaCO3) blend exhibiting the best overall performance. This blend achieved removal efficiencies of 81% for BOD, 83% for COD, 82% for TSS, 72% for nitrate, and 68% for phosphate. Furthermore, the pH of the treated effluent was stabilised within the range of 6.7–7.7, while dissolved oxygen increased significantly from 0.8 to 4.3 mg/L, indicating substantial improvement in water quality. The treated effluent approached the permissible limits specified by the World Health Organization (WHO) and the Nigerian Industrial Standards (NIS) for wastewater discharge. The findings demonstrate that blended AC–CaCO3 is an effective, economical, and environmentally sustainable adsorbent for the treatment of abattoir wastewater and offers a promising solution for improving wastewater management in resource-constrained settings.},
     year = {2026}
    }
    

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    Charcoal–Calcium Carbonate Adsorbent in the Treatment of Abattoir Wastewater
    AU  - Eshiole Lucky Oshiobugie
    AU  - Wasiu John
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    PB  - Science Publishing Group
    SN  - 2637-434X
    UR  - https://doi.org/10.11648/j.jeece.20261102.12
    AB  - The discharge of untreated abattoir wastewater poses a significant environmental and public health challenge due to its high concentrations of organic matter, suspended solids, nutrients, and other pollutants. Conventional treatment methods are often expensive and difficult to sustain in developing countries, creating the need for affordable and environmentally friendly alternatives. This study investigated the effectiveness of blended activated charcoal and calcium carbonate (AC–CaCO3) as a low-cost adsorbent for the treatment of abattoir effluent. Wastewater samples were collected from the Warake Road abattoir in Auchi, Edo State, Nigeria, and treated using AC–CaCO3 blends prepared in ratios of 1:1, 1:2 and 2:1. The treatment performance was evaluated by analysing physicochemical parameters, including pH, temperature, total suspended solids (TSS), total dissolved solids (TDS), turbidity, biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), nitrate, phosphate, and selected heavy metals. The results showed that the adsorption efficiency depended on the blending ratio, with the 1:2 (AC–CaCO3) blend exhibiting the best overall performance. This blend achieved removal efficiencies of 81% for BOD, 83% for COD, 82% for TSS, 72% for nitrate, and 68% for phosphate. Furthermore, the pH of the treated effluent was stabilised within the range of 6.7–7.7, while dissolved oxygen increased significantly from 0.8 to 4.3 mg/L, indicating substantial improvement in water quality. The treated effluent approached the permissible limits specified by the World Health Organization (WHO) and the Nigerian Industrial Standards (NIS) for wastewater discharge. The findings demonstrate that blended AC–CaCO3 is an effective, economical, and environmentally sustainable adsorbent for the treatment of abattoir wastewater and offers a promising solution for improving wastewater management in resource-constrained settings.
    VL  - 11
    IS  - 2
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Author Information
  • Department of Civil Engineering, Edo State University, Iyamho, Nigeria

  • Department of Civil Engineering, Edo State University, Iyamho, Nigeria

  • Department of Civil Engineering, Edo State University, Iyamho, Nigeria

  • Department of Civil Engineering, Edo State University, Iyamho, Nigeria

  • Abstract
  • Keywords
  • Document Sections

    1. 1. Introduction
    2. 2. Materials and Methods
    3. 3. Results and Discussion
    4. 4. Conclusion and Engineering Implications
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  • Abbreviations
  • Acknowledgments
  • Author Contributions
  • Conflicts of Interest
  • References
  • Cite This Article
  • Author Information