| Peer-Reviewed

The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk

Received: 13 November 2020    Accepted: 1 December 2020    Published: 11 December 2020
Views:       Downloads:
Abstract

Biotechnology is the application of scientific techniques to modify and improve plants, animals, and microorganisms to enhance their value. Genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. Transgenic technology deals with the integration of exogenous DNA into the plant genome using gene transfer technologies. Genetically modified (GM) crop plants contain artificially inserted gene (s) or “transgenes” from another unrelated plant or from a completely different species via advanced genetic engineering techniques. Transgenic crops do not present new categories of environmental risk compared to conventional methods of crop improvement. Transgenic plants can be generated using (1) biolistic technique where in the desired gene is coated on to either gold or tungsten particles are shot into plant cells using a gene-gun. The necessary criterion for this is that cells or plant tissues should be suitable for transformation permit regeneration of a whole plant thereafter. (2) The other major gene transfer technique makes use of the soil bacterium Agrobacterium tumefaciens, containing a tumor-inducing (Ti) plasmid including virulence (vir) gene (s) and a transferred-DNA (T-DNA) region, in which genes of interest can be inserted. Generally the gene that transfer from other organism to the desired crop (GM) can cause different environmental risks like, generation of super weed, development of tolerance to target herbicide, loss of biodiversity and sustainable resistance in insect pests, through gene flow. These risks can be minimized using different molecular techniques such as, maternal inheritance, male sterility, cleistogamy and apomixes, genetic use restriction technologies, genome incompatibility, controlling gene expression and transgenic mitigation.

Published in American Journal of Polymer Science and Technology (Volume 6, Issue 4)
DOI 10.11648/j.ajpst.20200604.11
Page(s) 32-39
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Environment, Genetically Modified Organism, Genetics, Molecular Technique, Transgenic Crop

References
[1] Allen, G. C., Spiker, S. and Thompson, W. F., 2005. Transgene integration: use of matrix attachment regions. In Transgenic Plants: Methods and Protocols (pp. 313-326). Humana Press.
[2] Alstad, D. N. and Andow, D. A., 1995. Managing the evolution of insect resistance to transgenic plants. Science, 268 (5219), pp. 1894-1896.
[3] Bock, R. and Khan, M. S., 2004. Taming plastids for a green future. TRENDS in Biotechnology, 22 (6), pp. 311-318.
[4] Bode, J., Benham, C., Knopp, A. and Mielke, C., 2000. Transcriptional augmentation: modulation of gene expression by scaffold/matrix-attached regions (S/MAR elements). Critical reviews in eukaryotic gene expression, 10 (1), pp. 73-90.
[5] Broothaerts, W., Mitchell, H. J., Weir, B., Kaines, S., Smith, L. M., Yang, W., Mayer, J. E., Roa-Rodriguez, C. and Jefferson, R. A., 2005. Gene transfer to plants by diverse species of bacteria. Nature, 433 (7026), p. 629.
[6] Cho, M. J., Yano, H., Okamoto, D., Kim, H. K., Jung, H. R., Newcomb, K., Le, V. K., Yoo, H. S., Langham, R., Buchanan, B. B. and Lemaux, P. G., 2004. Stable transformation of rice (Oryza sativa L.) via microprojectile bombardment of highly regenerative, green tissues derived from mature seed. Plant cell reports, 22 (7), pp. 483-489.
[7] Christou, P., 1995. Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment. Euphytica, 85 (1-3), pp. 13-27.
[8] Commandeur, U., Twyman, R. M. and Fischer, R., 2003. The biosafety of molecular farming in plants. AgBiotechNet, 5 (110), pp. 1-9.
[9] Conner, A. J., Glare, T. R. and Nap, J. P., 2003. The release of genetically modified crops into the environment: Part II. Overview of ecological risk assessment. The Plant Journal, 33 (1), pp. 19-46.
[10] Curtis, M. D., Brand, L., Yang, W., Nüesch, E., Moore, J. M., Jefferson, R. and Grossniklaus, U., 2004, September. Apomixis technology development: Transgene containment and fixation of heterosis. In 8th International Symposium on the Biosafety of Genetically Modified Organisms. Montpellier (pp. 167-173).
[11] Dale, P. J., Clarke, B. and Fontes, E. M., 2002. Potential for the environmental impact of transgenic crops. Nature biotechnology, 20 (6), p. 567.
[12] Daniell, H., 2002. Molecular strategies for gene containment in transgenic crops. Nature biotechnology, 20 (6), p. 581.
[13] Dove, A., 2001. Survey raises concerns about Bt resistance management.
[14] Dunwell, J. and Ford, C. S., 2005. Technologies for biological containment of GM and non-GM crops.
[15] Eckardt, N. A., 2006. Cytoplasmic male sterility and fertility restoration.
[16] Ellstrand, N. C., 2001. When transgenes wander, should we worry? Plant physiology, 125 (4), pp. 1543-1545.
[17] Engel, K. H., Frenzel, T. and Miller, A., 2002. Current and future benefits from the use of GM technology in food production. Toxicology letters, 127 (1-3), pp. 329-336.
[18] Esnayra, J., Pool, R. and National Research Council, 2000. Ecological monitoring of genetically modified crops: a workshop summary. National Academies Press.
[19] Gelvin, S. B., 1998. The introduction and expression of transgenes in plants. Current Opinion in Biotechnology, 9 (2), pp. 227-232.
[20] Gressel, J., 2002. Molecular biology of weed control. CRC Press.
[21] Haber, J. E., 2000. Recombination: a frank view of exchanges and vice versa. Current opinion in cell biology, 12 (3), pp. 286-292.
[22] Hails, R. S., 2000. Genetically modified plants–the debate continues. Trends in Ecology & Evolution, 15 (1), pp. 14-18.
[23] Hansen, G. and Wright, M. S., 1999. Recent advances in the transformation of plants. Trends in plant science, 4 (6), pp. 226-231.
[24] James, R. R., DiFazio, S. P., Brunner, A. M. and Strauss, S. H., 1998. Environmental effects of genetically engineered woody biomass crops. Biomass and Bioenergy, 14 (4), pp. 403-414.
[25] Konig, A., Cockburn, A., Crevel, R. W. R., Debruyne, E., Grafstroem, R., Hammerling, U., Kimber, I., Knudsen, I., Kuiper, H. A., Peijnenburg, A. A. C. M. and Penninks, A. H., 2004. Assessment of the safety of foods derived from genetically modified (GM) crops. Food and Chemical Toxicology, 42 (7), pp. 1047-1088.
[26] Kumar, S., Dhingra, A. and Daniell, H., 2004. Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant molecular biology, 56 (2), pp. 203-216.
[27] Lasa J. M., Bosemark N. O., 1993. Male sterility. In: Hayward M. D., Bosemark N. O., Romagosa I., Cerezo M. (eds) Plant Breeding. Plant Breeding Series. Springer, Dordrecht.
[28] Lee, D. and Natesan, E., 2006. Evaluating genetic containment strategies for transgenic plants. Trends in biotechnology, 24 (3), pp. 109-114.
[29] Lombardo, L., 2014. Genetic use restriction technologies: a review. Plant biotechnology journal, 12 (8), pp. 995-1005.
[30] Manasse, R. and Kareiva, P., 1991. Quantifying the spread of recombinant genes and organisms. Biotechnology (USA).
[31] Mayee, C. D., Singh, P., Dongre, A. B., Rao, M. R. K. and Raj, S., 2003. Transgenic Bt Cotton.
[32] Morikawa, H., Sakamoto, A., Hokazono, H., IRIFUNE, K. and TAKAHASHI, M., 2002. Mechanism of transgene integration into a host genome by particle bombardment. Plant biotechnology, 19 (4), pp. 219-228.
[33] National Research Council, 2002. Environmental effects of transgenic plants: the scope and adequacy of regulation. National Academies Press.
[34] Ohba, T., Yoshioka, Y., Machida, C. and Machida, Y., 1995. DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target. The Plant Journal, 7 (1), pp. 157-164.
[35] Pereira, A., 2000. A transgenic perspective on plant functional genomics. Transgenic research, 9 (4-5), pp. 245-260.
[36] Perr, H. A., 2002. Children and genetically engineered food: potentials and problems. Journal of pediatric gastroenterology and nutrition, 35 (4), pp. 475-486.
[37] Rommens, C. M., Humara, J. M., Ye, J., Yan, H., Richael, C., Zhang, L., Perry, R. and Swords, K., 2004. Crop improvement through modification of the plant's own genome. Plant Physiology, 135 (1), pp. 421-431.
[38] Ruf, S., Hermann, M., Berger, I. J., Carrer, H. and Bock, R., 2001. Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nature biotechnology, 19 (9), p. 870.
[39] S. Rastogi and N. Pathak, 2009. Genetic Engineering, Oxford University Press, New Delhi, India.
[40] Snow, A. A. and Palma, P. M., 1997. Commercialization of transgenic plants: potential ecological risks. BioScience, 47 (2), pp. 86-96.
[41] Traynor, P. L. and Westwood, J. H., 1999, January. Ecological effects of plant resistance genes in managed ecosystems. In Workshop Proceedings, Information Systems for Biotechnology (Vol. 31).
[42] Vielle-Calzada, J. P., Baskar, R. and Grossniklaus, U., 2000. Delayed activation of the paternal genome during seed development. Nature, 404 (6773), p. 91.
[43] Yan, L. and Kerr, P. S., 2002. Genetically engineered crops: their potential use for improvement of human nutrition. Nutrition Reviews, 60 (5), pp. 135-141.
[44] Zuo, J. and Chua, N. H., 2000. Chemical-inducible systems for regulated expression of plant genes. Current Opinion in Biotechnology, 11 (2), pp. 146-151.
[45] Ervin, D. E. and Welsh, R., 2006. Environmental effects of genetically modified crops: differentiated risk assessment and management. In Regulating agricultural biotechnology: Economics and policy (pp. 301-326). Springer, Boston, MA.
[46] Briefs, I. S. A. A. A., 2017. Global status of commercialized biotech/GM crops in 2017: Biotech crop adoption surges as economic benefits accumulate in 22 years.
[47] Babiye, B., Haile, G. and Adamu, M., 2020. Major Achievements of Plant Biotechnology in Crop Improvements. American Journal of Life Sciences, 8 (5), pp. 102-106.
Cite This Article
  • APA Style

    Girma Haile, Mulugeta Adamu, Tadele Tekle. (2020). The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk. American Journal of Polymer Science and Technology, 6(4), 32-39. https://doi.org/10.11648/j.ajpst.20200604.11

    Copy | Download

    ACS Style

    Girma Haile; Mulugeta Adamu; Tadele Tekle. The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk. Am. J. Polym. Sci. Technol. 2020, 6(4), 32-39. doi: 10.11648/j.ajpst.20200604.11

    Copy | Download

    AMA Style

    Girma Haile, Mulugeta Adamu, Tadele Tekle. The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk. Am J Polym Sci Technol. 2020;6(4):32-39. doi: 10.11648/j.ajpst.20200604.11

    Copy | Download

  • @article{10.11648/j.ajpst.20200604.11,
      author = {Girma Haile and Mulugeta Adamu and Tadele Tekle},
      title = {The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk},
      journal = {American Journal of Polymer Science and Technology},
      volume = {6},
      number = {4},
      pages = {32-39},
      doi = {10.11648/j.ajpst.20200604.11},
      url = {https://doi.org/10.11648/j.ajpst.20200604.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20200604.11},
      abstract = {Biotechnology is the application of scientific techniques to modify and improve plants, animals, and microorganisms to enhance their value. Genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. Transgenic technology deals with the integration of exogenous DNA into the plant genome using gene transfer technologies. Genetically modified (GM) crop plants contain artificially inserted gene (s) or “transgenes” from another unrelated plant or from a completely different species via advanced genetic engineering techniques. Transgenic crops do not present new categories of environmental risk compared to conventional methods of crop improvement. Transgenic plants can be generated using (1) biolistic technique where in the desired gene is coated on to either gold or tungsten particles are shot into plant cells using a gene-gun. The necessary criterion for this is that cells or plant tissues should be suitable for transformation permit regeneration of a whole plant thereafter. (2) The other major gene transfer technique makes use of the soil bacterium Agrobacterium tumefaciens, containing a tumor-inducing (Ti) plasmid including virulence (vir) gene (s) and a transferred-DNA (T-DNA) region, in which genes of interest can be inserted. Generally the gene that transfer from other organism to the desired crop (GM) can cause different environmental risks like, generation of super weed, development of tolerance to target herbicide, loss of biodiversity and sustainable resistance in insect pests, through gene flow. These risks can be minimized using different molecular techniques such as, maternal inheritance, male sterility, cleistogamy and apomixes, genetic use restriction technologies, genome incompatibility, controlling gene expression and transgenic mitigation.},
     year = {2020}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - The Effects of Genetically Modified Organisms (GMO) on Environment and Molecular Techniques to Minimize Its Risk
    AU  - Girma Haile
    AU  - Mulugeta Adamu
    AU  - Tadele Tekle
    Y1  - 2020/12/11
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajpst.20200604.11
    DO  - 10.11648/j.ajpst.20200604.11
    T2  - American Journal of Polymer Science and Technology
    JF  - American Journal of Polymer Science and Technology
    JO  - American Journal of Polymer Science and Technology
    SP  - 32
    EP  - 39
    PB  - Science Publishing Group
    SN  - 2575-5986
    UR  - https://doi.org/10.11648/j.ajpst.20200604.11
    AB  - Biotechnology is the application of scientific techniques to modify and improve plants, animals, and microorganisms to enhance their value. Genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. Transgenic technology deals with the integration of exogenous DNA into the plant genome using gene transfer technologies. Genetically modified (GM) crop plants contain artificially inserted gene (s) or “transgenes” from another unrelated plant or from a completely different species via advanced genetic engineering techniques. Transgenic crops do not present new categories of environmental risk compared to conventional methods of crop improvement. Transgenic plants can be generated using (1) biolistic technique where in the desired gene is coated on to either gold or tungsten particles are shot into plant cells using a gene-gun. The necessary criterion for this is that cells or plant tissues should be suitable for transformation permit regeneration of a whole plant thereafter. (2) The other major gene transfer technique makes use of the soil bacterium Agrobacterium tumefaciens, containing a tumor-inducing (Ti) plasmid including virulence (vir) gene (s) and a transferred-DNA (T-DNA) region, in which genes of interest can be inserted. Generally the gene that transfer from other organism to the desired crop (GM) can cause different environmental risks like, generation of super weed, development of tolerance to target herbicide, loss of biodiversity and sustainable resistance in insect pests, through gene flow. These risks can be minimized using different molecular techniques such as, maternal inheritance, male sterility, cleistogamy and apomixes, genetic use restriction technologies, genome incompatibility, controlling gene expression and transgenic mitigation.
    VL  - 6
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Department of Crop Production and Landscape Management, Ebonyi State University, Abakaliki, Nigeria

  • Department of Biotechnology, Mekelle University, Mekelle, Ethiopia

  • Department of Biology, Mizan-Tepi University, Mizan-Tepi, Ethiopia

  • Sections