Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi; Stephane Kevin Ndengue Langoul; Jean Michel Tekam; Pierre Rene Fotsing Kwetche

doi:doi:10.11648/j.ijpc.20220806.12

Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, Stephane Kevin Ndengue Langoul, Jean Michel Tekam, Pierre Rene Fotsing Kwetche

Published in International Journal of Pharmacy and Chemistry (Volume 8, Issue 6)

Received: 29 December 2022 Accepted: 16 January 2023 Published: 31 January 2023

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.

Published in	International Journal of Pharmacy and Chemistry (Volume 8, Issue 6)
DOI	10.11648/j.ijpc.20220806.12
Page(s)	75-81
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fresh Leaves Extract, Gardenia Aqualla, Polyphénols, Flavonoïds, Male Accessory Glands Infection

References

[1]	Ahsaini M, Omana JP, Mellas S, et al. Dysfonction érectile chez les patients diabétiques type 2, prévalence et gravité au Service d´ Urologie du Centre Hospitalier Universitaire de Fès, Maroc: à propos de 96 cas (étude transversale). Pan Afr Med J; 37.
[2]	Alechinsky L, Droupy S, Roupret M. Chapitre 09 - Trouble de l’érection. Association Française d’urologie, https://www.urofrance.org/congres-et-formations/formation-initiale/referentiel-du-college/trouble-de-lerection.html (2019, accessed 10 May 2021).
[3]	Bouhanick B, Blacher J, Huyghe E, et al. Dysfonction érectile et traitement antihypertenseur: impact des différentes classes thérapeutiques et conduite à tenir à l’égard du traitement. Presse Médicale 2019; 48: 1222–1228.
[4]	La Vignera S, Condorelli R, Vicari E, et al. High frequency of sexual dysfunction in patients with male accessory gland infections. Andrologia 2012; 44: 438–446.
[5]	Harvard Health Publishing, Havard Medical School. Sex and the Prostate: Overcoming erectile dysfunction when you have prostate disease. Harvard Health, https://www.health.harvard.edu/blog/sex-and-the-prostate-overcoming-erectile-dysfunction-when-you-have-prostate-disease-20090331100 (2009, accessed 16 September 2022).
[6]	Berthé HJG. Dysfonction érectile psychogène chez les patients mariés: A propos de 102 observations. Rev Afr Urol Androl; 1.
[7]	Zhou Y, Chen S, Zhang D, et al. The efficacy and safety of acupuncture in the treatment of erectile dysfunction: A protocol for systematic review and meta-analysis. Medicine (Baltimore) 2021; 100: e25892.
[8]	Nyalile KB, Mushi EH, Moshi E, et al. Prevalence and factors associated with erectile dysfunction among adult men in Moshi municipal, Tanzania: community-based study. Basic Clin Androl 2020; 30: 1–7.
[9]	Tavares IM, Moura CV, Nobre PJ. The role of cognitive processing factors in sexual function and dysfunction in women and men: a systematic review. Sex Med Rev 2020; 8: 403–430.
[10]	Boston University School of Medecine, Sexual medicine. Epidemiology of ED » Sexual Medicine » BUMC, https://www.bumc.bu.edu/sexualmedicine/physicianinformation/epidemiology-of-ed/ (2002, accessed 7 September 2022).
[11]	Ramlachan P. Erectile dysfunction, Sexual Health and Therapy, https://www.mydynamics.co.za/health_post/erectile-dysfunction-sexual-health-and-therapy/ (2021, accessed 7 September 2022).
[12]	Comeau D. La prévention de l’infertilité masculine. Médecin Qué 2002; 37: 75–81.
[13]	Grillo S, Cuervo G, Carratalà J, et al. Characteristics and Outcomes of Staphylococcus aureus Bloodstream Infection Originating From the Urinary Tract: A Multicenter Cohort Study. Open Forum Infect Dis; 7. Epub ahead of print June 2020. DOI: 10.1093/ofid/ofaa216.
[14]	Ogden NH, AbdelMalik P, Pulliam JRC. Maladies infectieuses émergentes: prévision et détection. Relevé Mal Transm Au Can 2017; 43: 232–8.
[15]	World Health Organisation. Antimicrobial resistance, https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance (2017, accessed 16 September 2022).
[16]	Ying A, Yu Q-T, Guo L, et al. Structural–activity relationship of ginsenosides from steamed ginseng in the treatment of erectile dysfunction. Am J Chin Med 2018; 46: 137–155.
[17]	Burkill HM. The useful plants of west tropical Africa: Families E-I. 2nd ed. Kew : United Kingdom: Royal Botanic Gardens, 2000.
[18]	Boyd R, Fournier A, Nignan S. Une base de données informatisée transdisciplinaire de la flore chez les Seme du Burkina Faso : un outil pour l’étude du lien nature-société Regards scientifiques croisés sur le changement global et le développement : langue, environnement, culture. In: In : Fabre G. (dir.), Fournier Anne (dir.), Sanogo L. (dir.) Regards scientifiques croisés sur le changement global et le développement : langue, environnement, culture. Ouagadougou: CNRST, 2014, pp. 165–199.
[19]	Wang LB, Wang JC, Friedman JM, et al. A case of epididymoorchitis without testicular infarction presenting with reversal of diastolic testicular flow on Doppler ultrasonography. Urol Case Rep 2021; 38: 101694.
[20]	Davis NG, Silberman. M. Bacterial Acute Prostatitis. [Updated 2022 Jul 17]. In: StatPearls. Treasure Island (FL): StatPearls Publishing, https://www.ncbi.nlm.nih.gov/books/NBK459257/ (2022).
[21]	Marconi M, Pilatz A, Wagenlehner F, et al. Impact of infection on the secretory capacity of the male accessory glands. Int Braz J Urol 2009; 35: 299–309.
[22]	Bao D, Xu X, Wang Y, et al. Emergence of a Multidrugs-Resistant Escherichia coli co-carrying a new mcr-l.33 variant and blaNDM-5 genes recovered from a unirary tract infection. Infect Drug Resist 2022; 15: 1499–1503.
[23]	Irfan M, Hussain NHN, Noor NM, et al. Epidemiology of male sexual dysfunction in Asian and European regions: a systematic review. Am J Mens Health 2020; 14: 1–40.
[24]	Bruneton J. Pharmacognosie, phytochimie, plantes médicinales (4e ed.). Lavoisier, 2009.
[25]	European Medicines Agency. ICH Topic Q 2 (R1): Validation of Analytical Procedures: Text and Methodology, https://www.ema.europa.eu/en/documents/scientific-guideline/ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf (2006, accessed 28 September 2020).
[26]	Carr GP, Wahlich JC. A practical approach to method validation in pharmaceutical analysis. J Pharm Biomed Anal 1990; 8: 613–618.
[27]	Belouafa S, Habti F, Benhar S, et al. Statistical tools and approaches to validate analytical methods: methodology and practical examples. Int J Metrol Qual Eng 2017; 8: 9.
[28]	Singleton VL, Rossi JA. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am J Enol Vitic 1965; 16: 144.
[29]	Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64: 555–559.
[30]	Kim D-O, Chun OK, Kim YJ, et al. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003; 51: 6509–6515.
[31]	Bonnet R, Caron F, Cavallo JD, et al. Comité de l’Antibiogramme de la Société Française de Microbiologie. Recomm Internet 2013; 19: 133–142.
[32]	Jehl F, Cattoen C. Comité de l’antibiogramme de la société française de microbiologie. Recomm 2016 V1 0 Février 2016; 117.
[33]	Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016; 6: 71–79.
[34]	Gayathiri E, Bharathi B, Priya K. Study of the enumeration of twelves clinical important bacterial populations at 0.5 McFarland standard. Int J Creat Res Thoughts 2018; 6: 880–893.
[35]	Faleyimu OI, Akinyemi O, Idris YM. Survey of forest plants used in traditional treatment of typhoid fever in Chikun Local Government Area of Kaduna State, Nigeria. Int J Biomed Health Sci; 6.
[36]	Ojo-Omoniyi OA, Osho RR, Odetunmibi OA, et al. Evaluation of the Microbial Flora and Shelf-Life Stability of Two Indigenous Palm Wine Products (Elaies guineensis and Raphia hookeri) Obtained from Southwest Nigeria. Int J Curr Microbiol App Sci 2020; 9: 1541–1558.
[37]	Ire FS, Okeke JI, Maduka N. Suitability and fermentative performance of indigenous palm wine yeast (Saccharomyces cerevisiae) using apple. South Asian J Biol Res 2020; 3: 55–74.
[38]	Penchev PI. Étude des procédés d’extraction et de purification de produits bioactifs à partir de plantes par couplage de techniques séparatives à basses et hautes pressions. Thèse de Doctorat, Université de Toulouse-Institut National Polytechnique de Toulouse, 2010.
[39]	Njinga NS, Sule MI, Pateh UU, et al. Phytochemical and Antimicrobial Activity of the Stem-Bark of Gardenia aqualla stapf & hutch (Rubeacea). J Med Plants Res 2014; 8: 942–946.
[40]	Nyemb JN, Magnibou LM, Talla E, et al. Lipids constituents from gardenia aqualla stapf & hutch. Open Chem 2018; 16: 371–376.
[41]	Muhammad BY, Shaban NZ, Elrashidy FH, et al. Antioxidant, Anti-inflammatory, Antiproliferative and Antimicrobial Activities of Combretum glutinosum and Gardenia aqualla Extracts in vitro. Free Radic Antioxid 2019; 9: 66–72.
[42]	Agbo MO, Osadebe PO, Noundou XS, et al. Antioxidant and Immunomodulatory Activities of Cameroonian Medicinal Plants. In: Natural Products Chemistry of Botanical Medicines from Cameroonian Plants. CRC Press, 2021, pp. 83–98.
[43]	Baan Munim T, Nazmul H. Bioactive secondary metabolites from plant sources: types, synthesis and their therapeutic uses. Int J Plant Biol 2022; 13: 4–14.
[44]	Le Floch R, Naux E, Arnould JF. L’infection bactérienne chez le patient brûlé. Ann Burns Fire Disasters 2015; 28: 94.
[45]	Meziani SE, Bedhouche N, Bouhoui C. Evaluation de l’activité antioxydante et de l’activité antibactérienne des extraits de Moringa oleifera.

Cite This Article

Plain Text BibTeX RIS

APA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. (2023). Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). International Journal of Pharmacy and Chemistry, 8(6), 75-81. https://doi.org/10.11648/j.ijpc.20220806.12

Copy | Download

ACS Style

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int. J. Pharm. Chem. 2023, 8(6), 75-81. doi: 10.11648/j.ijpc.20220806.12

Copy | Download

AMA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int J Pharm Chem. 2023;8(6):75-81. doi: 10.11648/j.ijpc.20220806.12

Copy | Download

@article{10.11648/j.ijpc.20220806.12,
  author = {Claude Berenger Ngantchouko Ngalemo and Gisele Laurel Tchiengang Tchoua and Francois Nicaise Bony and Blondin Gatien Tsawo and Patrick Yamen Mbopi and Stephane Kevin Ndengue Langoul and Jean Michel Tekam and Pierre Rene Fotsing Kwetche},
  title = {Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {8},
  number = {6},
  pages = {75-81},
  doi = {10.11648/j.ijpc.20220806.12},
  url = {https://doi.org/10.11648/j.ijpc.20220806.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220806.12},
  abstract = {Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)
AU  - Claude Berenger Ngantchouko Ngalemo
AU  - Gisele Laurel Tchiengang Tchoua
AU  - Francois Nicaise Bony
AU  - Blondin Gatien Tsawo
AU  - Patrick Yamen Mbopi
AU  - Stephane Kevin Ndengue Langoul
AU  - Jean Michel Tekam
AU  - Pierre Rene Fotsing Kwetche
Y1  - 2023/01/31
PY  - 2023
N1  - https://doi.org/10.11648/j.ijpc.20220806.12
DO  - 10.11648/j.ijpc.20220806.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 75
EP  - 81
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20220806.12
AB  - Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.
VL  - 8
IS  - 6
ER  -

Copy | Download

Author Information

Claude Berenger Ngantchouko Ngalemo

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Gisele Laurel Tchiengang Tchoua

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Francois Nicaise Bony

Department of Analytical, Mineral and General Chemistry, Food Technology, Training and Research Unit, Pharmaceutical and Biological Sciences, Félix Houphouet-Boigny University, Abidjan, C?te d’Ivoire
Blondin Gatien Tsawo

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Patrick Yamen Mbopi

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Stephane Kevin Ndengue Langoul

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Jean Michel Tekam

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Pierre Rene Fotsing Kwetche

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. (2023). Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). International Journal of Pharmacy and Chemistry, 8(6), 75-81. https://doi.org/10.11648/j.ijpc.20220806.12

Copy | Download

ACS Style

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int. J. Pharm. Chem. 2023, 8(6), 75-81. doi: 10.11648/j.ijpc.20220806.12

Copy | Download

AMA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int J Pharm Chem. 2023;8(6):75-81. doi: 10.11648/j.ijpc.20220806.12

Copy | Download

@article{10.11648/j.ijpc.20220806.12,
  author = {Claude Berenger Ngantchouko Ngalemo and Gisele Laurel Tchiengang Tchoua and Francois Nicaise Bony and Blondin Gatien Tsawo and Patrick Yamen Mbopi and Stephane Kevin Ndengue Langoul and Jean Michel Tekam and Pierre Rene Fotsing Kwetche},
  title = {Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {8},
  number = {6},
  pages = {75-81},
  doi = {10.11648/j.ijpc.20220806.12},
  url = {https://doi.org/10.11648/j.ijpc.20220806.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220806.12},
  abstract = {Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)
AU  - Claude Berenger Ngantchouko Ngalemo
AU  - Gisele Laurel Tchiengang Tchoua
AU  - Francois Nicaise Bony
AU  - Blondin Gatien Tsawo
AU  - Patrick Yamen Mbopi
AU  - Stephane Kevin Ndengue Langoul
AU  - Jean Michel Tekam
AU  - Pierre Rene Fotsing Kwetche
Y1  - 2023/01/31
PY  - 2023
N1  - https://doi.org/10.11648/j.ijpc.20220806.12
DO  - 10.11648/j.ijpc.20220806.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 75
EP  - 81
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20220806.12
AB  - Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.
VL  - 8
IS  - 6
ER  -

Copy | Download