Advances in Biochemistry

| Peer-Reviewed |

Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine

Received: Apr. 05, 2016    Accepted: Apr. 14, 2016    Published: Apr. 28, 2016
Views:       Downloads:

Share

Abstract

Carmoisine and tartrazine are azo dyes widely used as food colourants. This study examine the effects of oral administration of carmoisine and tartrazine on some biochemical parameters in the heart and kidney of albino rats. Twenty four (24) female albino rats (average weight 140g) were sorted into four groups. Group A received water and served as the control, Group B-250mg/kg bw carmoisine, Group C-250mg/kg bw tartrazine, Group D-co-administered 250mg/kg bw each of carmoisine and tartrazine. The food dyes were administered daily for a period of 21 days. Results obtained showed a significant increase (p<0.05) in the levels of urea, creatinine and electrolytes (Na+, K+ and CO32-) in the serum of rats administered the food dyes compared to control. The colourants also caused significant alterations (p<0.05) in the levels of enzymes (creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase) in the serum and heart homogenate of the experimental rats. Histological examination of the renal and cardiac tissues correlate with the biochemical results indicating marked distortions in the kidney and heart of rats administered carmoisine and tartrazine. These results suggest that carmoisine and tartrazine have the tendency of inducing nephrotoxicity and cardiac dysfunction in rats.

DOI 10.11648/j.ab.20160403.11
Published in Advances in Biochemistry ( Volume 4, Issue 3, June 2016 )
Page(s) 21-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Carmoisine, Tartrazine, Nephrotoxicity, Heart, Histology

References
[1] A. Downham and P. Collins. Colouring our foods in the last and next millennium. International Journal of Food Science and Technology. 2000, 35: 5–22.
[2] R. L. Newsome. Food colours. Food technology. 1986, 40 (7): 49-56.
[3] S. A. Soltan and E. M. Manal. The Effects of Using Colour Foods of Children on Immunity Properties and Liver, Kidney on Rats". Food and Nutrition Sciences. 2012, 3 (7): 897–904.
[4] J. Cook. Colorants compliance. The World of Food Ingredients Arnhem. The Netherlands. CNS Media. 2013, 41–43.
[5] H. Meggos. Food colours: an international perspective. The Manufacturing Confectioner. 1995, pp. 59–65.
[6] M. Donna. Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial. The Lancet. 2007, 370: 1560–1567.
[7] G. P. Ford, B. I. Stevenson and J. G. Evans. Long-term toxicity study of carmoisine in rats using animals exposed in vitro. Food Chem. Toxicol. 1987, 25 (12): 19–25.
[8] K. I. Golka, S. Kopps, H. M. Prager, S. V. Mende, R. Thiel, O. Jungmann, J. Zumbe, H. M. Bolt, M. Blaszkewicz, J. G. Hengstler and S. Selinski. Bladder cancer in crack testers applying azo dye-based sprays to metal bodies. J Toxicol and Environmental Health. 2012, 75 (8-10): 566-571.
[9] P. J. Wright, D. T. Plummer and P. T. Leathwood. Enzyme in rat urine. Alkaline phosphatase. Enzymologia. 1972, 42:317-327.
[10] S. Reitman and S. Frankel. A colorimetric method for the determination of serum glutamic oxaloacetate aminotransferase. Am. J. Clin. Pathol. 1957, 28:56-63.
[11] B. P. Hughes. A method for the estimation of serum creatine kinase and its use in comparing creatine kinase and aldolase activity in normal and pathological sera. Clin. chim. Acta, 1962, 7: 597.
[12] R. E. Vanderlinde. Measurement of total lactate dehydrogenase activities. Annals of Clinical Lab. Sci. 1985, 15 (1):13-31.
[13] D. R. D. Wybenga, J. Glorgio and V. J. Pileggi. Determination of serum urea by diacetyl monoxime method. J. Clin. Chem. 1971, 17: 891-895.
[14] R. J. Henry. Determination of serum creatinine. In: Clinical Chemistry: Principle and Technics. 2nd Edn. Harper and Row. 1974, p 525.
[15] N. W. Tietz, E. L. Pruden and O. Siggaard-Andersen. In: Tietz textbook of Clinical Chemistry (Burtis CA, Ashwell ER eds.) W.B Saunders Company London. 1994, 1354-1374.
[16] P. B. C. Friedman. Effect of diseases on clinical laboratory test. Am. Soc. Clin. Chem. J. 1980, 5(2):122-125.
[17] A. J. Patil, V. R. Bhagwat, J. A. Patil, N. N. Dongre, J.G. Ambekar and K. K. Das. Occupational lead exposure in battery manufacturing workers, silver jewelry workers and spray painters in Western Maharashtra (India): effects on liver and kidney function. J. Basic Clin. Physiol. Pharmacol. 2007, 18: 87-100.
[18] H. M. Wadei, M. L. Mai, N. Ahsan and T. A. Gonwa. Hepatorenal syndrome: Pathophysiology and management. Clin. J. Am. Soc. Nephro. 2006, 1 (5):1066-1079.
[19] J. S. Cameron and R. Greger. Renal function and testing of function. In Davison A. M, Cameron J. S., Grunfeld J. P., Kerr D. N. S., Rits E., Winearl G. C. eds Oxford textbook of Clinical Nephrology. 1998, Pp 36-39.
[20] S. R. Orth and E. Ritz. The nephritic syndrome. New England Journal of Medicine 1998, 338: 1202-1211.
[21] J. S. Bard. The kidney: from normal development to congenital disease. Boston: Academic Press. 2003, Pp.154.
[22] M. L. Halpperin and K. S. Kamel. Electrolyte Quinter: Potassium. Lancet. 1998, 352:135-140.
[23] A. A. Adesokan and M. A. Akanji. Effect of administration of aqueous extract of Enantia chlorantha on the activities of some enzymes in the small intestine of rats. Nig. J. Biochem. Mol. Biol. 2004, 18: 103-105.
[24] W. L. Clapp. Renal Anatomy. In: Zhou X. J., Laszik Z., Nadasdy T., D'Agati V. D., Silva F.G. eds. Silva's Diagnostic Renal Pathology. New York: Cambridge University Press. 2009.
[25] D. W. Moss and S.B. Rosalki. Enzyme tests in diagnosis. Edward Arnold. London. 1996, Pp. 68-77.
[26] N. S. Sorensen. Creatine phosphokinase in the diagnosis of myocardial infarction. Acta Med. Scand, 1963, 174,725.
[27] T. Wallimann, M. Wyss, D. Brdiczka, K. Nicolay and H. M. Eppenberger. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the phosphocreatine circuit for cellular energy homeostasis. The Biochemical Journal. 1992, 281(1): 21–40.
[28] C. Perry, H. Peretz, O. Ben-Tal, and A. Eldor. Highly elevated lactate dehydrogenase level in a healthy individual a case of macro-LDH American. J. Hematol, 1997, 55: 39–40.
[29] S. Neubauer. The Failing Heart: An Engine Out of Fuel. New England Journal of Medicine 2007, 356 (11): 1140–1151.
[30] M. G. Aymen. Introduction to functional and clinical histology, text and atlas, Part I, 4th ed. Giza, Egypt. 2000, 193-196.
Cite This Article
  • APA Style

    Olu Israel Oyewole, Johnson Olaleye Oladele. (2016). Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine. Advances in Biochemistry, 4(3), 21-25. https://doi.org/10.11648/j.ab.20160403.11

    Copy | Download

    ACS Style

    Olu Israel Oyewole; Johnson Olaleye Oladele. Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine. Adv. Biochem. 2016, 4(3), 21-25. doi: 10.11648/j.ab.20160403.11

    Copy | Download

    AMA Style

    Olu Israel Oyewole, Johnson Olaleye Oladele. Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine. Adv Biochem. 2016;4(3):21-25. doi: 10.11648/j.ab.20160403.11

    Copy | Download

  • @article{10.11648/j.ab.20160403.11,
      author = {Olu Israel Oyewole and Johnson Olaleye Oladele},
      title = {Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine},
      journal = {Advances in Biochemistry},
      volume = {4},
      number = {3},
      pages = {21-25},
      doi = {10.11648/j.ab.20160403.11},
      url = {https://doi.org/10.11648/j.ab.20160403.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ab.20160403.11},
      abstract = {Carmoisine and tartrazine are azo dyes widely used as food colourants. This study examine the effects of oral  administration of carmoisine and tartrazine on some biochemical parameters in the heart and kidney of albino rats. Twenty four (24) female albino rats (average weight 140g) were sorted into four groups. Group A received water and served as the control, Group B-250mg/kg bw carmoisine, Group C-250mg/kg bw tartrazine, Group D-co-administered 250mg/kg bw each of carmoisine and tartrazine. The food dyes were administered daily for a period of 21 days. Results obtained showed a significant increase (p+, K+ and CO32-) in the serum of rats administered the food dyes compared to control. The colourants also caused significant alterations (p<0.05) in the levels of enzymes (creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase) in the serum and heart homogenate of the experimental rats. Histological examination of the renal and cardiac tissues correlate with the biochemical results indicating marked distortions in the kidney and heart of rats administered carmoisine and tartrazine. These results suggest that carmoisine and tartrazine have the tendency of inducing nephrotoxicity and cardiac dysfunction in rats.},
     year = {2016}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Assessment of Cardiac and Renal Functions in Wistar Albino Rats Administered Carmoisine and Tartrazine
    AU  - Olu Israel Oyewole
    AU  - Johnson Olaleye Oladele
    Y1  - 2016/04/28
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ab.20160403.11
    DO  - 10.11648/j.ab.20160403.11
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
    SP  - 21
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2329-0862
    UR  - https://doi.org/10.11648/j.ab.20160403.11
    AB  - Carmoisine and tartrazine are azo dyes widely used as food colourants. This study examine the effects of oral  administration of carmoisine and tartrazine on some biochemical parameters in the heart and kidney of albino rats. Twenty four (24) female albino rats (average weight 140g) were sorted into four groups. Group A received water and served as the control, Group B-250mg/kg bw carmoisine, Group C-250mg/kg bw tartrazine, Group D-co-administered 250mg/kg bw each of carmoisine and tartrazine. The food dyes were administered daily for a period of 21 days. Results obtained showed a significant increase (p+, K+ and CO32-) in the serum of rats administered the food dyes compared to control. The colourants also caused significant alterations (p<0.05) in the levels of enzymes (creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase) in the serum and heart homogenate of the experimental rats. Histological examination of the renal and cardiac tissues correlate with the biochemical results indicating marked distortions in the kidney and heart of rats administered carmoisine and tartrazine. These results suggest that carmoisine and tartrazine have the tendency of inducing nephrotoxicity and cardiac dysfunction in rats.
    VL  - 4
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • Department of Biochemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria

  • Department of Biochemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria

  • Section