Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance.
| Published in | Pharmaceutical Science and Technology (Volume 5, Issue 1) |
| DOI | 10.11648/j.pst.20210501.13 |
| Page(s) | 14-23 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Coagulase Negative Staphylococcus, Efflux Proteins, Multiple Drug Resistant Organism, Phylogenetic Analysis
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APA Style
Kannan Balachander, Deborah Gnana Selvam Alexander. (2021). Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharmaceutical Science and Technology, 5(1), 14-23. https://doi.org/10.11648/j.pst.20210501.13
ACS Style
Kannan Balachander; Deborah Gnana Selvam Alexander. Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharm. Sci. Technol. 2021, 5(1), 14-23. doi: 10.11648/j.pst.20210501.13
AMA Style
Kannan Balachander, Deborah Gnana Selvam Alexander. Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharm Sci Technol. 2021;5(1):14-23. doi: 10.11648/j.pst.20210501.13
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Download@article{10.11648/j.pst.20210501.13,
author = {Kannan Balachander and Deborah Gnana Selvam Alexander},
title = {Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples},
journal = {Pharmaceutical Science and Technology},
volume = {5},
number = {1},
pages = {14-23},
doi = {10.11648/j.pst.20210501.13},
url = {https://doi.org/10.11648/j.pst.20210501.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.pst.20210501.13},
abstract = {Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance.},
year = {2021}
}
TY - JOUR T1 - Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples AU - Kannan Balachander AU - Deborah Gnana Selvam Alexander Y1 - 2021/05/26 PY - 2021 N1 - https://doi.org/10.11648/j.pst.20210501.13 DO - 10.11648/j.pst.20210501.13 T2 - Pharmaceutical Science and Technology JF - Pharmaceutical Science and Technology JO - Pharmaceutical Science and Technology SP - 14 EP - 23 PB - Science Publishing Group SN - 2640-4540 UR - https://doi.org/10.11648/j.pst.20210501.13 AB - Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance. VL - 5 IS - 1 ER -
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