Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract
Choudhary Dimple,
Naik Archana
Issue:
Volume 8, Issue 3, September 2022
Pages:
34-40
Received:
29 May 2022
Accepted:
22 June 2022
Published:
28 July 2022
DOI:
10.11648/j.jddmc.20220803.11
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Abstract: Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-urolithiasis activity and preparation of formulation. Flavonoid rich extract of Citrus medicawas prepared by maceration followed by liquid liquid extraction. Qualitative analysis was carried by TLC. Extract was then subjected to spray drying process to get free following powder for formulation. Standardization of flavonoid rich extract of fruit of CM was carried out by HPTLC method using toluene: ethyl acetate: formic acid (6:4:0.1 v/v) asmobile phaseand Silica gel GF 254nm as stationary phase with biomarker i.e. naringenin. Detection was carried out at 281 nm. Total flavonoid content was estimated by UV spectroscopy by considering quercetin as standard. Anti-urolithiasis activity was evaluated by in-vitro model using cystone as standard agent used for kidney stone. Extract showed potent activity as compared to standard. Spray dried powder of flavonoid rich extract was then incorporated in tablet formulation. Evaluation of tablet was carried out as per guidelines and it passes as per the specifications. Hence Citrus medica fruits which are traditionally popular is proved as potent agent as well as standardization give assurity about quality of the herbal formulation and can be used as potent anti-urolithiatic agent in ayurvedic era.
Abstract: Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-u...
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Physiological Infective Characteristics of JRCQC and TTRTF Durum Specific Pgt Races on Wheat Cultivars
Issue:
Volume 8, Issue 3, September 2022
Pages:
41-45
Received:
31 May 2022
Accepted:
6 July 2022
Published:
17 August 2022
DOI:
10.11648/j.jddmc.20220803.12
Downloads:
Views:
Abstract: Wheat is an important industrial grain crop which is nominated as strategic for food. Nowadays, wheat is also cultivated in lowlands. Ethiopia is endowed as wheat producing potential mainly at three regions in the country, SNNPRS, Oromia and Amhara regions are potential wheat producing regions. Productivity of wheat in Ethiopia 2.96 t/ha is lowervas compared to global average, 3.65 tha-1 it is due to poor Agronomic practices, poor farm managements, limited potential yielding variety, poor soil fertility, rain distribution, limited diseases and insects managements. So the objective is to search new sources of resistance gene for the selected stem rust races. The experiment was conducted at Ambo Agricultural Research Center (AmARC) in 2019 using 78 wheat variants (19 released commercial varieties, 23 landraces, 35 EBI genotypes and McNair as susceptible check). Among those tested 77 tested materials: Oda, Leliso, Asassa, Cocorit 71, LD-357, DW/LRce 1, DW/LRce 2, DW/LRce 3, DW/LRce 6, DW/LRce8, DW/LRce 17, DW/LRce 18, DW/LRce 15, DW/LRce 16, DW/LRce 17, DW/LRce 20), 203792, 231541, 226807, 204519, 226233, 208267, 210795 and 203790) had shown low infection type (IT:; fleck to 2) to TTRTF virulence race. The results indicated tested wheat cultivars have different levels of resistance to the tested races. The results enables by doing further research it provides; the development resistance varieties to stem rust.
Abstract: Wheat is an important industrial grain crop which is nominated as strategic for food. Nowadays, wheat is also cultivated in lowlands. Ethiopia is endowed as wheat producing potential mainly at three regions in the country, SNNPRS, Oromia and Amhara regions are potential wheat producing regions. Productivity of wheat in Ethiopia 2.96 t/ha is lowerva...
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