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Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.)

Received: 25 March 2022    Accepted: 25 April 2022    Published: 10 May 2022
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Abstract

The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.

Published in Bioprocess Engineering (Volume 6, Issue 1)
DOI 10.11648/j.be.20220601.11
Page(s) 1-4
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Elective Gels, Low Cost, Shoots Multiplication and Root Elongation

References
[1] Adel M. Al-Saif, A. B. M. Sharif Hossain and Rosna Mat Taha (2011) Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro. African Journal of Biotechnology Vol. 10 (27) 5291-5295.
[2] Berihu M., (2021) In vitro protocol optimization for mass propagation of pineapple (Ananas comosus L.) cv. smooth cayenne. European Journal of Biotechnology and Bioscience, 9 (2) 28-3.
[3] Escalona M. J. C. Lorenzo • B. González M. Daquinta • J. L. González • Y. Desjardins C. G. Borroto (1999) Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant Cell Reports 18: 743–748.
[4] Farahani F., (2014) micropropagation and growth of in vitro pineapple (ananas comosus l. merr) in iran. Plant Archives 14 (1) 337-341.
[5] Firoozabady, E. and Y. Moy (2004). Regeneration of pineapple plants via somatic embryogenesis and organogenesis. In Vitro Cell Dev. Biol. Plant, 40: 67-74.
[6] Food and Agriculture Foundation. (www.fao.org) Guo, B., Bilal, H. A., Amir, Z., Xu, L. L. & Wei, Y. H. 2010. Thidiazuron: A multi-dimensional plant growth regulator. African Journal Biotechnology. 10 (45): 8984–9000.
[7] George, E. F. (1996). Plant propagation by tissue culture part-II: In practice (pp. 761–786). Edington: Exegetics Ltd.
[8] Henderson WE, Kinnersley AM (1988). Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell Tissue Organ Cult., 15: 17-22.
[9] MacLachlan J (1985). Macroalgae (Seaweeds): Industrial sources and their utilizations. Plant Soil, 89: 137-157.
[10] MacLeod, K., & Nowak, J. (1990). Glass beads as a solid matrix in in vitro study of the role of polyamines in cold hardiness of white clover. Plant Cell Tissue and Organ Culture, 22, 113–117.
[11] Mengesha, A., Ayenew, B., & Tadesse, T. (2013). Acclimatization of in vitro propagated pineapple (Ananas comosuss (L.), var. smooth cayenne) plantlets to ex vitro condition in Ethiopia. Ajps 10 (2): 2-9.
[12] Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
[13] Perrin RM, Young LS, Murthy UMN, Harrison BR, Wang Y, Will JL, Masson PH (2005). Gravity signal transduction in primary roots. Ann. Bot. 96: 737-743.
[14] Thorpe TA (2007). History of plant tissue culture. Mol. Biotechnol. 37: 169-180.
[15] Waisel Y, Eshel A, Kafkafi U (2002). Plant Roots. The Hidden Half (3rd Edition). Marcel, Dekker, Inc. New York Basel.
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  • APA Style

    Berihu Mengs Gebrehiwet. (2022). Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.). Bioprocess Engineering, 6(1), 1-4. https://doi.org/10.11648/j.be.20220601.11

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    ACS Style

    Berihu Mengs Gebrehiwet. Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.). Bioprocess Eng. 2022, 6(1), 1-4. doi: 10.11648/j.be.20220601.11

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    AMA Style

    Berihu Mengs Gebrehiwet. Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.). Bioprocess Eng. 2022;6(1):1-4. doi: 10.11648/j.be.20220601.11

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  • @article{10.11648/j.be.20220601.11,
      author = {Berihu Mengs Gebrehiwet},
      title = {Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.)},
      journal = {Bioprocess Engineering},
      volume = {6},
      number = {1},
      pages = {1-4},
      doi = {10.11648/j.be.20220601.11},
      url = {https://doi.org/10.11648/j.be.20220601.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20220601.11},
      abstract = {The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.)
    AU  - Berihu Mengs Gebrehiwet
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    PY  - 2022
    N1  - https://doi.org/10.11648/j.be.20220601.11
    DO  - 10.11648/j.be.20220601.11
    T2  - Bioprocess Engineering
    JF  - Bioprocess Engineering
    JO  - Bioprocess Engineering
    SP  - 1
    EP  - 4
    PB  - Science Publishing Group
    SN  - 2578-8701
    UR  - https://doi.org/10.11648/j.be.20220601.11
    AB  - The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.
    VL  - 6
    IS  - 1
    ER  - 

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Author Information
  • Plant Biotechnology Lab, Jimma Agricultural Research Center, Jimma, Ethiopia; Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia

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