Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens

Tumwesige Mshobozi Katto; Gaymary George Bakari; Amandus Muhairwa

doi:doi:10.11648/j.ijast.20250904.14

Research Article |

| Peer-Reviewed

Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens

Tumwesige Mshobozi Katto^*

, Gaymary George Bakari

, Amandus Muhairwa

Published in International Journal of Animal Science and Technology (Volume 9, Issue 4)

Received: 14 September 2025 Accepted: 26 September 2025 Published: 22 November 2025

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Abstract

This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p < 0.05). These improvements were comparable to those observed in the AGP-supplemented group, however, no significant differences were observed between them (p >0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.

Published in	International Journal of Animal Science and Technology (Volume 9, Issue 4)
DOI	10.11648/j.ijast.20250904.14
Page(s)	207-221
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Broiler, Prebiotics, Probiotics, Eubiotics, Performance, Intestinal- histomorphometry

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Katto, T. M., Bakari, G. G., Muhairwa, A. (2025). Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. International Journal of Animal Science and Technology, 9(4), 207-221. https://doi.org/10.11648/j.ijast.20250904.14

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Katto, T. M.; Bakari, G. G.; Muhairwa, A. Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. Int. J. Anim. Sci. Technol. 2025, 9(4), 207-221. doi: 10.11648/j.ijast.20250904.14

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Katto TM, Bakari GG, Muhairwa A. Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. Int J Anim Sci Technol. 2025;9(4):207-221. doi: 10.11648/j.ijast.20250904.14

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@article{10.11648/j.ijast.20250904.14,
  author = {Tumwesige Mshobozi Katto and Gaymary George Bakari and Amandus Muhairwa},
  title = {Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens
},
  journal = {International Journal of Animal Science and Technology},
  volume = {9},
  number = {4},
  pages = {207-221},
  doi = {10.11648/j.ijast.20250904.14},
  url = {https://doi.org/10.11648/j.ijast.20250904.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20250904.14},
  abstract = {This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p 0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.
},
 year = {2025}
}

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TY  - JOUR
T1  - Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens

AU  - Tumwesige Mshobozi Katto
AU  - Gaymary George Bakari
AU  - Amandus Muhairwa
Y1  - 2025/11/22
PY  - 2025
N1  - https://doi.org/10.11648/j.ijast.20250904.14
DO  - 10.11648/j.ijast.20250904.14
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 207
EP  - 221
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20250904.14
AB  - This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p 0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.

VL  - 9
IS  - 4
ER  -

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Author Information

Tumwesige Mshobozi Katto

Department of Animal Sciences, Mwalimu Nyerere University of Agriculture and Technology, Musoma (HQ-Butiama), Tanzania; Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Morogoro, Tanzania

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http://orcid.org/0009-0004-3029-5329
Gaymary George Bakari

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Morogoro, Tanzania

Contact Email

http://orcid.org/0000-0001-6514-5938
Amandus Muhairwa

Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences, Morogoro, Tanzania

Contact Email

http://orcid.org/0000-0001-6166-4538

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APA Style

Katto, T. M., Bakari, G. G., Muhairwa, A. (2025). Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. International Journal of Animal Science and Technology, 9(4), 207-221. https://doi.org/10.11648/j.ijast.20250904.14

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ACS Style

Katto, T. M.; Bakari, G. G.; Muhairwa, A. Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. Int. J. Anim. Sci. Technol. 2025, 9(4), 207-221. doi: 10.11648/j.ijast.20250904.14

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AMA Style

Katto TM, Bakari GG, Muhairwa A. Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens. Int J Anim Sci Technol. 2025;9(4):207-221. doi: 10.11648/j.ijast.20250904.14

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@article{10.11648/j.ijast.20250904.14,
  author = {Tumwesige Mshobozi Katto and Gaymary George Bakari and Amandus Muhairwa},
  title = {Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens
},
  journal = {International Journal of Animal Science and Technology},
  volume = {9},
  number = {4},
  pages = {207-221},
  doi = {10.11648/j.ijast.20250904.14},
  url = {https://doi.org/10.11648/j.ijast.20250904.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20250904.14},
  abstract = {This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p 0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.
},
 year = {2025}
}

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TY  - JOUR
T1  - Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens

AU  - Tumwesige Mshobozi Katto
AU  - Gaymary George Bakari
AU  - Amandus Muhairwa
Y1  - 2025/11/22
PY  - 2025
N1  - https://doi.org/10.11648/j.ijast.20250904.14
DO  - 10.11648/j.ijast.20250904.14
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 207
EP  - 221
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20250904.14
AB  - This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p 0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.

VL  - 9
IS  - 4
ER  -

Copy | Download