Research Article
Phenotypic and Genotypic Characterization of Aspergillus uessalvadorensis in an Organic Strain Discovered at the University of El Salvador 2006 - 2024
Antonio Vásquez Hidalgo*
Issue:
Volume 13, Issue 1, March 2025
Pages:
1-16
Received:
21 January 2025
Accepted:
12 February 2025
Published:
18 March 2025
DOI:
10.11648/j.plant.20251301.11
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Abstract: Morphological characterization and molecular DNA techniques allowed the identification of the Aspergillus sample sent to MACROGEN SOUTH KOREA. Objective: To perform phenotypic and genotypic characterization of the genus Aspergillus by the Next-Generation Sequencing method (NGS). Methodology: The type of study is exploratory and experimental. It was carried out in two phases: first in the collection of seeds of Caesalpinia coriaria and second an initial macroscopic and microscopic characterization of the isolation of Aspergillus was carried out in 2006, a notarial act was carried out in 2007, studies of simple microscopy and scanning electron microscopy and PCR of the fungus found in Mexico 2008 and published in the journal La Universidad in 2008, then the extraction of gDNA, qPCR, cDNA was performed in 2024 at MACROGEN INC. by Metagenome Shotgun Sequencing Reports. Results: The gDNA genome was extracted obtaining a maximum concentration of 12.297 ng/ul, volume 30 ul, total amount 0.369 and DIN 6.4 maximum level 8732 sample intensity for 15000 bp, the quantum qDNA was obtained at 624 bp at a concentration of 103.24 nM and 41.87 ng/ul and cDNA. From the gDNA extraction of the TapeStation gDNA Screen, a maximum concentration of 12.297 ng/ul, volume 30 ul, total quantity 0.369 and DIN 6.4 maximum level 8732 sample intensity for 15000 bp in quality control was obtained. qPCR 624 bp were obtained at a concentration of 103.24 nM and 41.87 ng/ul with the TruSeq Nano DNA library (350_META). cDNA library 33 library kits were used. Total, of bases obtained were 11,705,895,990 bp, total, of readings were 77,522,490. GC nucleotide content % 49.7 and AT % 50.30, GC base content was 49.7% and AT was 50.3, Metric base content Q 20: 95.1 and Q 30: 88.3, Q30 cycle data quality high value. FASTQ and FASTA formats were used for encoding and full base-pair sequencing. Raw data Value. 38,761.245 N, adapter quality and trimming. (Quality and adapter trim.) was 32,535,420, Elimination of contaminants was 30,961,740. From the Krona taxonomy, Aspergillus was reported. Heatmaps specie report varieties. Conclusions: Aspergillus sp was found by the NGS sequencing method with a variety of species, in previous studies in 2006 it was named the species uessalvadorensis.
Abstract: Morphological characterization and molecular DNA techniques allowed the identification of the Aspergillus sample sent to MACROGEN SOUTH KOREA. Objective: To perform phenotypic and genotypic characterization of the genus Aspergillus by the Next-Generation Sequencing method (NGS). Methodology: The type of study is exploratory and experimental. It was...
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