Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation
L. Y. Yang,
X. Y. Guo,
Y. Q. Di,
Y. Sun
Issue:
Volume 4, Issue 4, July 2016
Pages:
23-28
Received:
25 August 2016
Accepted:
5 September 2016
Published:
22 September 2016
Abstract: Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.
Abstract: Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the...
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