American Journal of Environmental Protection

Volume 9, Issue 2, April 2020

  • The Development and Application of DDPCR Technology on Quantification of Total Coliforms in Water

    Wei Ma, Yi Jun Kong, Weng U Ho, Si Ian Lam, Gui Huan Liu, Sin Neng Chio

    Issue: Volume 9, Issue 2, April 2020
    Pages: 22-30
    Received: 15 April 2020
    Accepted: 30 April 2020
    Published: 14 May 2020
    Abstract: In this research, the detection method for absolute quantification of total coliforms was established based on Droplet Digital Polymerase Chain Reaction (DDPCR) technology using lacZ as the target gene for coliform group detection. The experimental conditions (e.g. primer and probe concentrations, annealing temperatures, etc) were well optimized. B... Show More
  • Threats and Conservation Strategies on Urban Wetlands: A Case of Monavale and Surrounding Areas in Harare, Zimbabwe

    Silipiwe Sharai, Tarakini Tawanda, Chibememe Gladman

    Issue: Volume 9, Issue 2, April 2020
    Pages: 31-38
    Received: 20 April 2020
    Accepted: 5 May 2020
    Published: 27 May 2020
    Abstract: Wetlands are valuable ecosystems on which humans depend through the utilisation of the ecological goods and services including water for domestic and commercial use. Until recently, efforts on wetland conservation have shifted to community participation since they are important stakeholders in using wetland resources. This study solicited local peo... Show More